Work

Predicting temozolomide response in low-grade glioma patients with large-scale machine learning

Year: 2025

Type: article

Abstract: ABSTRACT Background Temozolomide is the primary chemotherapeutic agent and first-line treatment for low-grade glioma (LGG). Although LGGs are generally less aggressive than high-grade gliomas (HGGs), they can eventually progress into HGGs, making it crucial to maximise the efficacy of initial LGG treatment. Methods In this study, we analysed data from 109 LGG patients in The Cancer Genome Atlas (TCGA) to assess the predictive performance of 12 machine learning (ML) classification algorithms in forecasting temozolomide response, utilising six types of omics data. Cross-validation and bootstrapping bias correction were employed to compare the performance of these models with that of a conventional biomarker-based model using MGMT promoter methylation status. Results Among the models, the miRNA-based approach using the XGBoost algorithm showed the most promising predictive performance, with a Matthews Correlation Coefficient (MCC) of 0.447, outperforming the auto-ML method JADBio (MCC = 0.250) and the MGMT biomarker-based model. Converting the task into a regression framework by encoding cancer response as a continuous variable generally weakened predictive performance, with best model based on methylation profile (MCC = 0.344). Additionally, ML models focusing on biomarker-relevant features demonstrated improved prediction accuracy, though not to the extent of omics-based models, with the support vector machine (SVM) model achieving an MCC of 0.331. Incorporating clinical variables, such as patient age and Karnofsky score, further enhanced predictive power, with the LR-OMC model achieving the highest MCC (0.483). Feature importance analysis identified six significant miRNA factors, including three tumour-related miRNAs (miR-335, let-7f, and miR-7-2) and three potential biomarkers (miR-204, miR-6513, and miR-376). Discussion Overall, this study systematically demonstrates the potential of large-scale analyses combining machine learning and omics data to predict temozolomide response, offering superior predictive accuracy compared to standard MGMT biomarkers.

Source: BMC Methods

DOI: 10.1186/s44330-025-00046-3

Authors: Hanqin Du, Chayanit Piyawajanusorn, Ghita Ghislat, Pedro J. Ballester

Institution: Imperial College London

Language: English

Topic: Glioma Diagnosis and Treatment

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,490