Extraction, Identification and Hypoglycemic and Lipid-lowering Activity of Polyphenols from Aronia melanocarpa
Year: 2025
Type: article
Abstract: The extraction process, composition and hypoglycemic and lipid-lowering activity of Aronia melanocarpa polyphenols (AMP) extracted by ultrasonic-assisted deep eutectic solvents were investigated. The optimal process was obtained by single factor experiment and Box-Behnken response surface method. The AMP components purified by macroporous resin were identified and analyzed by ultra performance liquid chromatograph mass spectrometer (UPLC-QTof-MS/MS). Finally, the inhibitory ability of α-glucosidase and pancreatic lipase was determined by simulating the digestibility of gastrointestinal tract in vitro. The results showed that the molar ratio of DES-2 (choline chloride-lactic acid) was 1:3, the water content was 80%, the ultrasonic power was 240 W, and the ultrasonic time was 40 min. The highest yield of AMP was 51.27 mg/g. There were 45 components in the purified AMP extracted by DES, including 15 anthocyanins, 3 proanthocyanidins, 18 flavonoids, and 9 phenolic acids. The total content was 116.92±7.54 mg/g, and its composition and content were significantly higher than those extracted by traditional organic solvents. After oral, gastric and intestinal digestion, the inhibition rate of AMP purified product on α-glucosidase reached 30.4%±1.2%, which was 3.34 times that of blueberry polyphenol purified product (P<0.05), and the inhibition rate of pancreatic lipase reached 92.7%±2.9%, which was 1.04 times that of blueberry polyphenol purified product (P<0.05). Compared with traditional organic solvents, DES extraction of AMP has high extraction efficiency, high yield, no pollution, many components and strong activity, and has certain feasibility of food industry application.
Authors: Dan GUO, Yan SUN, Chengzhong LI, Saikun PAN, Ling LUAN, Xingpu QI, Baoyuan ZHAO
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7084037460
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