A Biosensor Based on Commercial R-SAW for Rapid and Sensitive Detection of E. coli
Year: 2025
Type: article
Abstract: Escherichia coli (E. coli) is one of the most common strains that produce Shiga toxin, which can contaminate food and water, causing serious diseases and even endangering life. Therefore, the detection of E. coli is crucial for protecting public health. At present, most traditional methods have disadvantages such as long detection cycles, high cost, and complex operations. This article proposed a novel commercial Rayleigh surface acoustic wave (R-SAW) biosensor for the detection of trace amounts of E. coli, which utilized the coordination reaction between carboxyl (-COOH) groups and aluminum ions (Al3+) to form the bio-enhanced probes, enabling the 5-terminal -COOH-modified aptamers to be preferentially enriched and directionally immobilized on the electrode surface. The biosensor could complete the detection within 100 s, with a linear detection range of 103–108 cells/mL, a limit of detection (LOD) as low as 732 cells/mL, and a selectivity ratio of 3270:1. This article conducted spiked detection on six types of food, indicating that the biosensor had the advantages of rapid speed, high sensitive, wide detection range, low LOD, strong specificity, and low cost, providing an economical and convenient solution for detecting trace amounts of E. coli in food.
Source: Chemosensors
Authors: Li Lv, Zhuoer Xu, Yicheng Zhou, Jayne Wu, Xueyong Zhang, Haochen Qi
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,413
OpenAlex ID: W7084063625