Investigation of Antioxidant Enzymes (GST, GSH, R-GSSG) in Erythrocytes of COVID-19 Patients: A One-Month Observation
Year: 2025
Type: article
Abstract: Elżbieta Cecerska-Heryć,1 Natalia Serwin,1 Ewa Pius-Sadowska,2 Klaudia Głowacka,1 Aleksandra Polikowska,1 Małgorzata Goszka,1 Radosław Birger,1 Marta Budkowska,3 Bogusław Machaliński,2 Barbara Dołęgowska1 1Department of Laboratory Medicine, Pomeranian Medical University of Szczecin, Szczecin, Poland; 2Department of General Pathology, Pomeranian Medical University of Szczecin, Szczecin, Poland; 3Department of Analytical Medicine Pomeranian Medical University of Szczecin, Szczecin, PolandCorrespondence: Elżbieta Cecerska-Heryć, Email [email protected]: Oxidative stress (OS) occurs when there is an imbalance between reactive oxygen species (ROS) production and the bodyâs ability to neutralize them. This can lead to cellular damage and is associated with aging and various diseases. Glutathione (GSH) acts as a vital non-enzymatic antioxidant, while enzymes such as glutathione S-transferase (GST) and glutathione reductase (GR) help maintain the bodyâs redox balance. OS from viruses, including coronavirus, can disrupt inflammation regulation and cause prolonged tissue damage.Aim: This study examines GSH levels and the activities of GST and R-GSSG in the erythrocytes of COVID-19 patients compared to those of healthy controls.Materials and Methods: The study involved 85 COVID-19 patients (42 females and 43 males), with blood samples collected at the time of disease detection and at 7, 14, and 28 days later. The control group included 85Â healthy individuals. Antioxidant levels (GSH, GST, R-GSSG) were measured using spectrophotometric methods.Results: COVID-19 patients exhibited a significant decrease in GSH concentration (p < 0.001) and an increase in R-GSSG (p < 0.001) and GST (p = 0.046) activity. Lower GSH levels correlated with a higher risk of death (p = 0.008). Multivariate analysis revealed relationships between GSH and factors such as time since detection, survival rate, hospital stay, and disease severity.Conclusion: COVID-19 disrupts antioxidant enzyme balance, potentially worsening disease severity and increasing mortality risk, especially in older patients.Keywords: antioxidant enzymes, GST, GSH, R-GSSG oxidative stress, COVID-19
Authors: Cecerska-HeryÄ,Elżbieta, Serwin,Natalia, Pius-Sadowska,Ewa, GÅowacka,Klaudia, Polikowska,Aleksandra, Goszka,MaÅgorzata, Birger,RadosÅaw, Budkowska,Marta, MachaliÅski,BogusÅaw, DoÅÄgowska,Barbara
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7084199135
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