Year: 2025
Type: preprint
Abstract: This protocol describes the process of the hybridization of P5 and P7 adapters for the Blunt-End-Single-Tube (BEST) library build protocol. Following this protocol produces the P5+P7 adapter complex for use in the BEST library build. This protocol is based on: Carøe, C., Gopalakrishnan, S., Vinner, L., T. Mak, S. S., S. Sinding, M. H., Samaniego, J. A., Wales, N., Sicheritz-Pontén, T., & P. Gilbert, M. T. (2018). Single-tube library preparation for degraded DNA. Methods in Ecology and Evolution, 9(2), 410-419. https://doi.org/10.1111/2041-210X.12871 Always cite the original paper when using this protocol, in addition to citing this protocols.io protocol.
Institution: University of Copenhagen
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7084395097
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