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Research progress of Class 2 CRISPR-Cas system in nucleic acid detection of animal pathogens

Year: 2025

Type: article

Abstract: Novel overview of Class 2 CRISPR-Cas systems for veterinary diagnostics.• Comparative analysis of Cas9, Cas12, and Cas13 detection platforms.• Integration with isothermal amplification enables rapid, ultrasensitive assays.• Multiplex readouts improve on-site applicability and diagnostic accuracy.• Future directions highlight amplification-free and field-deployable CRISPR tools.

Source: Frontiers of Agricultural Science and Engineering

DOI: 10.15302/j-fase-2025659

Author: Shuai ZHANG, Xi SHEN, Zhongzhi LIU, Debao HU, Xin LI, Yiwen GUO, Xiangbin DING, Linlin ZHANG

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: diamond

OpenAlex ID: W7088472280

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