Expression of Micro RNA192 in Type 2 Diabetes Mellitus Relation to Glycemic Control, Metabolic Abnormalities, Renal and Ocular Complications
Year: 2013
Type: article
Study design: Observational Study
Abstract: Background: MicroRNAs are a class of naturally occurring small noncoding RNAs that regulate gene expression, cell growth, differentiation and apoptosis by targeting mRNAs for translational repression or cleavage. miRNAs associated with various pathological conditions including DM. Microvascular disease is the most frequent complication of diabetes. It is responsible for diabetic retinopathy nephropathy, and neuropathy. Purpose: This work was carried out to evaluate the expression level of miRNA 192 in type 2 diabetic patients and to assess the relation of miRNA 192 expression to glycemic control, metabolic abnormalities, renal and ocular complications aiming at early diagnosis and prevention of these complications. Patients and Methods: This study was conducted on 36 type 2 diabetic patients subdivided into 2 main groups according to onset of DM. 16 newly diagnosed cases and 20 patients with long standing DM as compared to 14 (age and sex matched) healthy persons. Patients with long standing DM further subdivided into diabetic nephropathy and patients with diabetic retinopathy. Patients were subjected to: detailed history taking and clinical examination, and laboratory investigation. The expression levels of miRNA192 in whole blood using (RT-PCR) were determined. Results: Mean expression level of miRNA 192 was significantly higher in diabetics compared to healthy subjects. This increase is more obvious for long standing diabetics than newly diagnosed diabetics. Similar findings were also noted for patients with diabetic retinopathy and nephropathy. Patients with diabetic nephropathy showed higher expression level of miRNA 192 as compared to diabetic patients with retinopathy. There was association between miRNA 192 and disease duration; there was significant positive correlation between miRNA 192 expression levels and blood glucose, HbA1c, creatinine and urinary albumin excretion rate in diabetic nephropathy subgroup. There was a significant positive correlation between miRNA 192 expression levels and blood glucose, HbA1c in patients with diabetic retinopathy. Conclusion: The study concluded that increase expression of miRNA 192 in type 2 DM, metabolic abnormalities, poor glycemic control and obesity, are risk factors related to prevalence of renal and ocular complications among diabetic subjects. Our results suggest that miRNA 192 may serve not only as molecular signatures in diabetic microvascular complications but also as early indicators of alterations in specific biological processes in the kidney, retina, and nervous system. We recommend further studies to evaluate the diagnostic, prognostic value of miRNA 192 and to evaluate anti-miRNA 192 as a therapeutic option in various diabetic microvascular complications.
Source: American Journal of Biochemistry
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7089878