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Green Synthesis of Cobalt Oxide (π‘ͺπ’πŸ‘π‘ΆπŸ’ ) Nanoparticles by Using Citrus Sinensis Peel Extract for Antibacterial Treatment

Year: 2025
Type: article
Abstract: In this study, cobalt oxide nanoparticles were synthesized through green method using 0.25𝑀 cobalt nitrate hexahydrate as a precursor salt and peel waste extract of citrus sinensis as a reducing and capping agent. The synthesis process of πΆπ‘œ3𝑂4 NPs were performed with in three various volume ratios of the precursor salt and the extract of the peel waste citrus sinensis as 1 ∢ 2 ( 33.3π‘šπ‘™ solution of 0.25M cobalt nitrate hexahydrate ∢ 66.7π‘šπ‘™ of 30g peel extract), 1 ∢ 1 ( 50π‘šπ‘™ solution of 0.25M cobalt nitrate hexahydrate ∢ 50π‘šπ‘™ of 30g peel extract), and 2 ∢ 1 ( 66.7π‘šπ‘™ solution of 0.25M cobalt nitrate hex hydrate ∢ 33.3π‘šπ‘™ of 30g peel extract).The synthesized πΆπ‘œ3𝑂4 NPs were characterized using X-Ray diffraction, scanning electron microscope, Ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. The average crystal size and purity of the green formed πΆπ‘œ3𝑂4 NPs were found to be 22.14 π‘›π‘š,11.19 π‘›π‘š, and 14.58 π‘›π‘š, respectively for the 2 ∢ 1 , 1 ∢ 1 and 1 ∢ 2, respectively. The band gap energy was 3.77𝑒𝑉,3.70𝑒𝑉 and 3.55𝑒𝑉 for the 1 ∢ 1,1 ∢ 2, and 2 ∢ 1, respectively. The Fourier transform infrared spectroscopy study proves the presence of different functional groups and bioactive capping and reducing. The antibacterial efficiency of synthesized πΆπ‘œ3𝑂4 NPs was tested for antibacterial test Staphylococci Aureus and Escherichia coli using 50πœ‡π‘”/π‘šπ‘™,75πœ‡π‘”/π‘šπ‘™, and 100πœ‡π‘”/π‘šπ‘™ concentration. πΆπ‘œ3𝑂4 (2 ∢ 1) shows zone of inhibition of 8, 9,10 and 9, 10 and 11 π‘šπ‘š for Escherichia coli and Staphylococci Aureus, respectively. 𝐼𝑓 πΆπ‘œ3𝑂4 (1: 1) shows zone of inhibition of 7, 13, 15 and 7, 14 and 17π‘šπ‘š against Escherichia coli and Staphylococci Aureus. Finally, πΆπ‘œ3𝑂4 ( 1 ∢ 2 ) with the same concentration showed zone of inhibition of 9, 11, 12 and 9, 11, 13π‘šπ‘š against Escherichia coli and Staphylococci Aureus, respectively
Author: ERMIASE YETSEDW
Language: English
Open Access status: green
OpenAlex ID: W7090766564
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