Advances in Microscopy Techniques for Biochemical Interactions and Cellular Imaging
Year: 2025
Type: article
Abstract: Recent technological advances in microscopy have revolutionized biochemical and cellular research by enabling visualization of molecular interactions and dynamic processes at nanometer resolution. Super-resolution techniques such as STED, PALM/STORM, and MINFLUX now permit real-time tracking of protein complexes and enzymatic activities within living cells. Correlative light and electron microscopy (CLEM) merges molecular specificity with ultrastructural context, unlocking new insights into cellular architecture and biochemical pathways. Integration of artificial intelligence and machine learning further accelerates image analysis, allowing automated segmentation, multiplexing, and high-throughput studies. Emerging multimodal platforms combining fluorescence, electron, and vibrational imaging promise comprehensive biochemical mapping at unprecedented scales. This review consolidates recent breakthroughs, addressing current challenges and outlining a roadmap for future innovations poised to propel cell biology, drug discovery, and precision medicine into new frontiers of understanding and discovery.
Author: Mohammad Javed*1, Sita Kumari2, Twinkle Gupta3, Smriti Gandha1
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7091335366
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