Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease.
Year: 2002
Type: article
Abstract: Most techniques that identify antigen-specific T cells are dependent on the response of these cells to the relevant antigen in culture. Soluble multimers of MHC molecules, when occupied with the same peptide, will bind selectively to T cells specific for that MHC/peptide complex. Techniques to produce fluorescent MHC class II/peptide multimers have recently been developed. These reagents provide a method to facilitate detection and isolation of antigen-specific CD4+ T cells and they represent a new research tool to study these cells in patients with immune-mediated diseases.
Source: Arthritis Research
DOI: 10.1186/ar417
Authors: Jerome R Bill, Brian L Kotzin
Institution: National Jewish Health
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1603162349