Work

VariantSalmonellaGenomic Island 1 Antibiotic Resistance Gene Cluster inSalmonella entericaSerovar Albany

Year: 2003

Type: article

Abstract: ApCmFfSmSpSuTcTm, ApSu, SmSpSu, SmSpSuTm, and ApSmSpSuTc, respectively (15).We examined a strain of S. enterica serovar Albany, isolated from food fish from Thailand and imported in France, that displayed the multidrug-resistance profile ApCmFfSuTcTm.This multidrug-resistance profile suggested the possible occurrence of SGI1 with a new variant antibiotic resistance cluster in this serovar. Materials and MethodsThe S. enterica serovar Albany strain 7205.00 used in this study was isolated from a food fish from Thailand.This strain and control strains S. Typhimurium DT 104 BN9181 (8,13,14), S. Agona 959SA97 (8,13,14), S. Paratyphi B 44 (14), and Escherichia coli strain TOP10 (Invitrogen SARL, Cergy-Pontoise, France) used in cloning experiments were grown at 37°C in brain heart infusion broth or agar plates.The strains were tested for their antibiotic susceptibility by the disc-diffusion assay on Mueller-Hinton plates.Susceptibility was tested by using discs containing the following antibiotics: Ap (10 µg), Cm (30 µg), Ff (30 µg), Sm (10 IU), Sp (100 µg), Su (200 µg), Tc (30 IU), and Tm (5 µg).All antibiotic disks except for Ff were purchased from Bio-Rad (Marnes-la-Coquette, France).Ff disks and the drug itself were obtained from Schering-Plough Animal Health (Kenilworth, NJ).MICs of Ff and Cm were determined by using the standard agar doubling dilution method.MIC breakpoints for Cm and Ff were defined by the Comité de l'Antibiogramme de la Société Française de Microbiologie (CASFM) or by the manufacturer (i.e., susceptible [MIC <8 µg/mL], intermediate [MIC = 16 µg/mL], or resistant [MIC >32 µg/mL]).

Source: Emerging infectious diseases

DOI: 10.3201/eid0905.020609