Potential of fecal microbiota for early‐stage detection of colorectal cancer
Year: 2014
Type: article
Study design: Observational Study
Abstract: Several bacterial species have been implicated in the development of colorectal carcinoma (CRC), but CRC-associated changes of fecal microbiota and their potential for cancer screening remain to be explored. Here, we used metagenomic sequencing of fecal samples to identify taxonomic markers that distinguished CRC patients from tumor-free controls in a study population of 156 participants. Accuracy of metagenomic CRC detection was similar to the standard fecal occult blood test (FOBT) and when both approaches were combined, sensitivity improved > 45% relative to the FOBT, while maintaining its specificity. Accuracy of metagenomic CRC detection did not differ significantly between early- and late-stage cancer and could be validated in independent patient and control populations (N = 335) from different countries. CRC-associated changes in the fecal microbiome at least partially reflected microbial community composition at the tumor itself, indicating that observed gene pool differences may reveal tumor-related host-microbe interactions. Indeed, we deduced a metabolic shift from fiber degradation in controls to utilization of host carbohydrates and amino acids in CRC patients, accompanied by an increase of lipopolysaccharide metabolism.
Source: Molecular Systems Biology
Authors: Georg Zeller, Julien Tap, Anita Y Voigt, Shinichi Sunagawa, Jens Roat Kultima, Paul I Costea, Aurélien Amiot, Jürgen Böhm, Francesco Brunetti, Nina Habermann, Rajna Hercog, Moritz Koch, Alain Luciani, Daniel R Mende, Martin A Schneider, Petra Schrotz‐King, Christophe Tournigand, Jeanne Tran Van Nhieu, Takuji Yamada, Jürgen Zimmermann, Vladimir Benes, Matthias Kloor, Cornelia M Ulrich, Magnus von Knebel Doeberitz, Iradj Sobhani, Peer Bork
Institutions: European Molecular Biology Laboratory, Université Paris-Est Créteil, European Molecular Biology Laboratory, German Cancer Research Center, University Hospital Heidelberg, National Center for Tumor Diseases, Assistance Publique – Hôpitaux de Paris, Tokyo Institute of Technology, Fred Hutch Cancer Center, Max Delbrück Center
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $7,990
OpenAlex ID: W2130725058