Metabolic Signatures of Exercise in Human Plasma

Year: 2010

Type: article

Abstract: Exercise provides numerous salutary effects, but our understanding of how these occur is limited. To gain a clearer picture of exercise-induced metabolic responses, we have developed comprehensive plasma metabolite signatures by using mass spectrometry to measure >200 metabolites before and after exercise. We identified plasma indicators of glycogenolysis (glucose-6-phosphate), tricarboxylic acid cycle span 2 expansion (succinate, malate, and fumarate), and lipolysis (glycerol), as well as modulators of insulin sensitivity (niacinamide) and fatty acid oxidation (pantothenic acid). Metabolites that were highly correlated with fitness parameters were found in subjects undergoing acute exercise testing and marathon running and in 302 subjects from a longitudinal cohort study. Exercise-induced increases in glycerol were strongly related to fitness levels in normal individuals and were attenuated in subjects with myocardial ischemia. A combination of metabolites that increased in plasma in response to exercise (glycerol, niacinamide, glucose-6-phosphate, pantothenate, and succinate) up-regulated the expression of nur77, a transcriptional regulator of glucose utilization and lipid metabolism genes in skeletal muscle in vitro. Plasma metabolic profiles obtained during exercise provide signatures of exercise performance and cardiovascular disease susceptibility, in addition to highlighting molecular pathways that may modulate the salutary effects of exercise.

Source: Science Translational Medicine

DOI: 10.1126/scitranslmed.3001006

Institutions: Broad Institute, Harvard University, Massachusetts General Hospital, Beth Israel Deaconess Medical Center, Brigham and Women's Hospital, Framingham Heart Study, Boston University

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Human Genome Research Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke

Awards: Clinical Evaluation of Novel Markers of MI, G250621538, G327723646, MIGRAINE DRUG PROPHYLAXIS, G4666976601, G5164707915, The Framingham Heart Study, Individual Propensity to Venous Thrombosis, Computational Methods for Directed Functional Genomics, Metabolic Profiling of Acute Myocardial Injury in Humans, Genomic Analysis of Network Perturbations in Human Disease, Metabolomic predictors of insulin resistance and diabetes

OpenAlex ID: W2171282332