Work

Joining Forces: Integrating Proteomics and Cross-linking with the Mass Spectrometry of Intact Complexes

Year: 2011

Type: review

Abstract: Protein assemblies are critical for cellular function and understanding their physical organization is the key aim of structural biology. However, applying conventional structural biology approaches is challenging for transient, dynamic, or polydisperse assemblies. There is therefore a growing demand for hybrid technologies that are able to complement classical structural biology methods and thereby broaden our arsenal for the study of these important complexes. Exciting new developments in the field of mass spectrometry and proteomics have added a new dimension to the study of protein-protein interactions and protein complex architecture. In this review, we focus on how complementary mass spectrometry-based techniques can greatly facilitate structural understanding of protein assemblies.

Source: Molecular & Cellular Proteomics

DOI: 10.1074/mcp.r111.014027

Authors: Florian Stengel, Ruedi Aebersold, Carol V. Robinson

Institutions: ETH Zurich, University of Oxford, Institute for Molecular Systems Biology, University of Zurich

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funder: European Commission