Work

Applicability of RNA standards for evaluating RT-qPCR assays and platforms

Year: 2011

Type: article

Abstract: The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

Source: BMC Genomics

DOI: 10.1186/1471-2164-12-118

Authors: Alison S Devonshire, Ramnath Elaswarapu, Carole A Foy

Institution: LGC

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,090

Funder: National Institute of Standards and Technology