Work

Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization

Year: 2015

Type: article

Abstract: Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH₂-Alginate, is synthesized through an oxidation-amination-reduction process. The "catalysis function" ensures the as-prepared NH₂-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na₂SiO₃), and the "template function" cause microscopic phase separation in aqueous solution. The diameter of resultant NH₂-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH₂-Alginate. The size and morphology of obtained NH₂-Alginate/silica nanocomposite are correlated with the micelles. NH₂-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH₂-Alginate and cage effect of silica matrix.

Source: Materials

DOI: 10.3390/ma8095286

Authors: Jian Li, Jun Ma, Tao Jiang, Yanhuan Wang, Xuemei Wen, Guozhu Li

Institutions: Tianjin University of Science and Technology, Tianjin Synthetic Material Research Institute (China), Tianjin University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,137

Funders: National Natural Science Foundation of China, Tianjin University, Natural Science Foundation of Tianjin City