Work

Towards direct visualization of the reaction coordinates of proteins

Year: 2025

Type: conference-abstract

Abstract: As for man-made machines, the functional cycles of enzymes and other proteins involve the coordinated motion of their parts. These motions, and the mechanical properties of proteins more generally, therefore link protein structure, function, and evolution. For many proteins, experimental access to protein mechanics remains elusive. I will describe our efforts to access these dynamics by electric field-stimulated X-ray crystallography and complementary methods. I will illustrate this approach using two examples: our work on the direct visualization of K+ ion permeation through an ion channel, and on the dissection of structural intermediates enzymatic cycle of dihydrofolate reductase.

Source: Structural Dynamics

DOI: 10.1063/4.0001050

Authors: Doeke R Hekstra, BoRam Lee, Rama Ranganathan, Kristopher I White, Margaret A. Klureza, Jack B. Greisman, Kevin M. Dalton, Robert W Henning, Vukica Srajer

Institutions: Harvard University, University of Chicago, Stanford University, D. E. Shaw Research, SLAC National Accelerator Laboratory, New York University

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,200

OpenAlex ID: W7101443060

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