Work

СRISPR-TTP Integrated Bioengineering Architecture: Complete Clinical Implementation Protocol v1.0

Year: 2025

Type: article

Abstract: Сomplete Standard Operating Procedures (SOP) for CRISPR-TTP Integrated Bioengineering Architecture designed for Ewing Sarcoma treatment. This document provides full technical specifications for clinical implementation including: • GMP-compliant cell processing protocols • Nanoparticle formulation (27±3 nm, PDI<0.15) • CRISPR editing parameters (1.8 μM Cas9, 94% efficiency) • 21-day clinical implementation timeline • AI-driven optimization framework • Safety monitoring protocols License: CC0 1.0 Universal - Public Domain Dedication

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.17537569

Author: Alexey Mikhailovich

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7104024249

FWCI:

Cites:

Cited by:

Related to: