Minimizing Cytotoxic Effects on Normal Cells: Enhanced Targeted Delivery of Docetaxel via PCL-PEG-PCL Nanocarriers in Breast Cancer Treatment
Year: 2025
Type: article
Abstract: Nanoparticle-based drug delivery systems are rapidly advancing in cancer therapy, providing reduced toxic side effects and improved efficacy through controlled drug release. This study encapsulated docetaxel (DTX) in a PCL-PEG-PCL matrix using a modified nano-precipitation method, focusing on its cytotoxic effects on breast cancer cell lines. The research aimed to clarify the mechanisms behind the cytotoxic effects and cell death induced by DTX-loaded nano-carriers, utilizing human breast cancer cell lines as an experimental model. The synthesized nano-carriers were characterized through methods such as transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). Drug release kinetics and encapsulation efficiency were evaluated with high-performance liquid chromatography (HPLC) and dialysis techniques. The cytotoxicity of DTX-loaded nano-carriers and free DTX was assessed in MCF-7, MDA-MB-231, and fibroblast cell lines over 48 and 72 hours using MTT assays to measure viability and Hoechst 33342 staining for apoptosis. Results showed that nano-carriers significantly inhibited cell growth in MCF-7 and MDA-MB-231 cells, especially with formulation P1 over 72 hours, compared to 48 hours. Apoptosis assays confirmed the effective induction of cell death by nano-carriers. The IC50 values for formulation P1 were substantially higher after 72 hours. Overall, these findings suggest that the designed nano-carriers promote controlled drug release and enhance apoptotic cell death while reducing the toxic side effects of docetaxel on normal cells, highlighting their potential in cancer therapeutics.
Authors: Ali Khojastehfar, Amrollah Mostafazadeh, Ebrahim Zabihi, Hadi Nataj, Pejman Hakemi, Ghasem Janbabaei, Mahdi Pouramir
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7105182363
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