Development and validation of q-absorbance ratio method for simultaneous quantification of linagliptin and metformin HCl in synthetic mixture and combined tablets
Year: 2025
Type: article
Abstract: The present study aims to develop and validate simple, cost effective Q-Absorbance Ratio Method for simultaneous quantification of Linagliptin (LIN) and Metformin HCl (MET). The proposed medium Acetonitrile: Methanol: Distilled Water at 1:1:1 (Solvent Blend) identified λmax 235nm for MET, 298nm for LIN and Isobestic point at 231.7nm for Q-Absorbance Ratio Method. The validation follows ICH Q2(R1) guidelines, demonstrating excellent linearity, accuracy, precision and sensitivity (LOD and LOQ) for LIN and MET. The developed method was successfully applied to a synthetic mixture and marketed formulation, showing % recovery within the acceptable limit with % RSD was less than 2.
Authors: Vaishnav, Rishika, Vasavi, Vasavi, Srinivasulu, N, Kumar, Y. Anand
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W7105808546
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