Work

Comparison of DNA-seq and RNA-seq in plant microbiome identification on rye – a case study

Year: 2025

Type: article

Abstract: High-throughput sequencing of RNA is an indispensable tool in plant virology for virus detection and identification. However, the acquired dataset consists of more than just viral reads and traces of other pathogens can be detected in the data, like bacteria and fungi. Using RNA-seq for detection of more than just viral pathogens have been discussed in literature.To test the reliability in detection of various microbes using only RNA-seq, this study presents the comparison of microorganisms detected in DNA-seq and RNA-seq analysis of the same samples. Five symptomatic and one asymptomatic (no visible symptoms) rye samples were collected from the field. DNA and RNA were extracted from the same plant material.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.17725159

Authors: Niedzicka, Marta, Kildea, Steven, Byrne, Stephen

Institution: Teagasc - The Irish Agriculture and Food Development Authority

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funder: European Commission

Award: G8289621968