Developments of Mycorrhiza in Quercus acutissimar (Cork Oak) Seedlings Inoculated with Black Truffle (Tuber melanosporum) Mycelial Pure Cultures
Year: 2025
Type: article
Abstract: Tuber melanosporum (T. melanosporum), which is known as the black truffle, is the most famous edible ectomycorrhizal (ECM) fungus with a great commercial interest in the world, due to the expensive delicacies and can develop symbiotically with the roots of various trees and some bushes. T. melanosporum was ascomycete hypogeous fungi, which forms an underground fruitbody. In this study, we infected the seedlings of Quercus acutissimar (cork oak), which is a main forest species grown naturally in DPR Korea, with T. melanosporum mycelium to identify the emerged mycorrhizal association morphologically and molecularly. It was shown that Q. acutissimar, selected as the host species, was compatible with T. melanosporum. One hundred of the Q. acutissimar seeds were allowed to germinate and germination was observed in 84 seeds. Among 84 germinated seedlings, 70 seedlings with relatively good growth were selected and inoculated with T. melanosporum mycelium. Characteristic mycorrhizal structures between T. melanosporum and Quercus acutissimar were observed in 52 seedlings. The identification of T. melanosporum mycorrhiza was based on morphological and anatomical features and confirmed with molecular analyses by PCR. This is the first report on the establishment of the ECM association between T. melanosporum mycelia with Quercus acutissimar seedlings in DPR Korea.
Authors: Kum-dong Jang, Yong-nam Kim, Chol Kim, Bok-sil Hyon, Hyok Kim, Hyok Kim, Jong-chong Kim, Hui-won Kim, Hui-won Kim
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7108225235