Arabidopsis nanopore direct RNA sequencing data for mutants in splicing, polyadenylation, and m6A methylation
Year: 2026
Type: data-paper
Abstract: Co-transcriptional RNA processing, including alternative splicing and polyadenylation, generates diverse mRNA isoforms essential for development, differentiation, and stress responses. We present Oxford Nanopore Direct RNA Sequencing (DRS) data from Arabidopsis thaliana wild-type (Col-0) plants and mutants defective in RNA processing pathways, including polyadenylation, RNA methylation, and pre-mRNA splicing. We obtained full-length reads together with native poly(A) tail information. The provided resources include FAST5 and FASTQ files, poly(A) tail length estimates from Nanopolish, poly(A) composition data from Ninetails, and differential expression analyses generated with DESeq2, all aligned to the TAIR10 reference transcriptome. These data offer a comprehensive view of RNA processing at the single-molecule level and can be reused to explore alternative polyadenylation, RNA modification patterns, or indirectly, splicing alterations across various genetic backgrounds. This resource is intended to support studies of gene expression regulation and RNA metabolism in Arabidopsis thaliana and related plant systems.
Source: Scientific Data
Authors: Natalia Gumińska, Łukasz Szewc, Agnieszka Czarnocka-Cieciura, Wiktoria Orzeł, Sebastian Jeleń, Mateusz Bajczyk, Seweryn Mroczek, Andrzej Dziembowski, Artur Jarmołowski
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,490
OpenAlex ID: W7108340340