Work

The Microbial Community Succession Drives Stage-Specific Carbon Metabolic Shifts During Agaricus bisporus Fermentation: Multi-Omics Reveals CAZymes Dynamics and Lignocellulose Degradation Mechanisms

Year: 2025

Type: article

Abstract: This study integrates metagenomic and metabolomic data to systematically analyze the microbial community succession and carbon source metabolism transitions during the third fermentation cycle of Agaricus bisporus, with the aim of optimizing fermentation efficiency and lignocellulose degradation strategies. Principal Coordinate Analysis (PcoA) based on Bray–Curtis dissimilarity reveals significant microbial community separation across the stages of the first mushroom fruiting cycle. The overall pattern of “stage-specific differentiation” is consistent with the “cellulose hydrolysis” turn to the degradation of complex polysaccharides via carbohydrate-active enzymes (CAZymes). In the microbial network analysis showed that different microbe group controlled the stage-specific differentiation. These findings highlight a phase-dependent metabolic shift during the fermentation process: the early stages of fruiting rely more on cellulose-degrading microbes and their enzymatic systems, while later stages are driven by the degradation of complex polysaccharides and lignin derivatives, with Planctomycetota leading the degradation. This provides new mechanistic insights into agricultural waste resource utilization and the directional regulation of fermentation processes.

Source: Microorganisms

DOI: 10.3390/microorganisms13122755

Authors: Chaozheng Wang, Yicheng Yu, Weilin Feng, Yuwei Xu, Tianju Deng, Weiming Cai, Wusheng Liang, Hongkai Wang

Institutions: Zhejiang University, ZheJiang Academy of Agricultural Sciences

Language: English

Topic: Fungal Biology and Applications

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,258