Work

Sucrose accumulation and key sucrose synthase gene screening in ‘early beauty’ plum fruit across different developmental stages

Year: 2025

Type: article

Abstract: ‘Early Beauty’ is an elite early-maturing plum (Prunus salicina L.) cultivar. However, the dynamic changes in sucrose accumulation and the key regulatory genes involved in sucrose biosynthesis remain largely unknown. In this study, high-resolution spatiotemporal datasets were obtained using GC–MS/MS combined with RNA-seq to systematically investigate the dynamics of sucrose metabolism and transcriptional landscapes in ‘Early Beauty’ plum fruit throughout development. The results revealed that sucrose is the predominant soluble sugar at fruit maturity, with the period from 59 to 66 DAFB identified as the critical phase for sucrose accumulation. The PsSY0025487 was selected for further study using RNA-seq. Phylogenetic analysis showed highest homology to peach PpSS1, designated it PsSS1. Overexpression of PsSS1 increased sucrose content by 85.6 % (from 23.71 to 44.02 mg/g), while silencing reduced it by approximately 42 %, demonstrating its critical role in sucrose accumulation. This study not only elucidates the spatiotemporal patterns of sucrose metabolism in ‘Early Beauty plum, but supports metabolic regulatory research in plum and other fruit crops.

Source: Scientia Horticulturae

DOI: 10.1016/j.scienta.2025.114541

Authors: Xueping Han, Huimin Tan, Qinglin Niu, Ran Dong, Xiaomin Xue

Institution: Shandong Agricultural University

Language: English

Topic: Postharvest Quality and Shelf Life Management

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,000