Effects of methylmercury exposure on the transcriptomes of cell lines from the rat central nervous system
Year: 2025
Type: article
Abstract: Methylmercury (MeHg) can have profound effects on cells of the nervous system, leading to DNA damage, oxidative stress, neuroinflammation, and cell death. In this study, transcriptomic changes in neuronal (B103) and glial (C6) cell lines from rat ( Rattus norvegicus ) were investigated after exposure to non-lethal concentrations of MeHg. In neuronal cells, low MeHg concentration (0.1 µM) affected signaling pathways related to protein metabolism and signal transduction, including those involving glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) and 3β-hydroxysteroid dehydrogenase (3β-HSD). Conversely, high MeHg concentration (2.8 µM) primarily disrupted ion transport pathways (e.g. SLC-mediated transmembrane transport) and oxidative stress responses. In glial cells, a low concentration of MeHg (0.1 µM) impaired the Rho-GTPase pathway, while a higher concentration (6.3 µM) impaired sodium–potassium-exchange-ATPase (Na+/K+-ATPase) activity. MicroRNA profiling showed that MAPK signaling was impaired by MeHg in both cell lines regardless of exposure level, with the mmu-miR-466 family consistently involved. Furthermore, ontology analysis of human phenotypes linked these transcriptomic changes to abnormalities in the nervous system and neurological development, establishing a link between MeHg exposure and disturbances in brain morphology and cognitive impairment. These findings elucidate critical molecular mechanisms underlying MeHg-induced neurotoxicity and underscore the far-reaching implications for cellular function and central nervous system health.
Source: Discover Toxicology
Authors: Laís Teixeira Bonfim, Carolina Koury Nassar Amorim, Edivaldo Herculano Corrêa de Oliveira, Wallax Augusto Silva Ferreira
Institutions: Universidade Federal do Pará, Instituto Evandro Chagas
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,340
OpenAlex ID: W7109064151