Balancing the tradeoffs in IVT: enzyme engineering, reaction optimization, and dsRNA control for safe, scalable RNA production
Year: 2025
Type: article
Abstract: “The growing demand for RNA-based vaccines and therapeutics has exposed critical trade-offs in current in vitro transcription (IVT) workflows. To ensure safe and scalable RNA production, manufacturers must balance enzyme engineering, reaction optimization, and dsRNA control to improve efficiency without compromising quality.”
Source: Vaccine Insights
Authors: Kaixi Zhao, Emily Wen
Institution: Merck & Co., Inc., Rahway, NJ, USA (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7111237155