Work

Yersinia ruckeri YRB periplasmic binding protein YiuA selectively recognizes a Fe(III)-mono-catecholate siderophore

Year: 2025

Type: article

Abstract: The marine pathogen Yersinia ruckeri synthesizes the tricatecholate siderophore ruckerbactin, Rb, (DHB-LArg-LSer)3, to acquire iron during infection. Its biosynthetic gene cluster encodes a single periplasmic binding protein, RupB, which surprisingly does not bind Fe(III)-Rb nor the Fe(III) complexes of its hydrolysis products, the di- and mono-catecholate siderophores RbDC and RbMC, with biologically relevant affinities. Instead, the periplasmic binding protein YiuA, encoded in a different region of the chromosome, binds the 1:2 Fe(III) complex of the mono-catecholate RbMC, Fe(III)-(RbMC)2. YiuA is the first periplasmic binding protein (PBP) to selectively recognize a mono-catecholate siderophore, the structural basis of which was illuminated through X-ray crystallography of YiuA bound to Fe(III)-(RbMC)2.

Source: White Rose Research Online (University of Leeds, The University of Sheffield, University of York)

Authors: GROGAN, GIDEON JAMES, Thomsen, Emil, THOMPSON, SEÁN, Stow, Parker, Cukor, Maren, DUHME-KLAIR, ANNE, Butler, Alison

Institutions: University of Leeds, University of Sheffield

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green