AAV production and purification: key steps from design to GMP readiness
Year: 2025
Type: article
Abstract: AAV manufacturing presents challenges across both upstream and downstream processes, each requiring optimization to facilitate robust and scalable production. The following studies focus on AAV production workflow and how different steps within the upstream and downstream processes were optimized. Namely, cell expansion, plasmid transfection, and vector production in the upstream process, and purification and analytics in the downstream process. Case studies highlight how critical raw materials and process parameters were chosen to enable productivity and scalability as well as how to meet the quality target product profile of the AAV produced.
Source: Cell and Gene Therapy Insights
Authors: Goutham Ganjam, Emily Jackson-Holmes, Florian Leseigneur, Sravanthi Pasupuleti, Marco Wachowius, Helena Martins, Eduard Ayuso
Institution: Thermo Fisher Scientific (United States)
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7114782669
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