Work

Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

Year: 2025

Type: article

Abstract: Caspase 3 signaling pathways using Western blot and enzyme-linked immunosorbent assay (ELISA). DAPI staining was used to visualize cell death in hAMSCs-treated Panc1 cells. The results showed an up-regulation of TNF-α, IL-1β, IL-8, p-IKK, p-IKKα, p-IKKβ, p-IκB, p53, PUMA, Caspase 3, and NF-κB (p50/p65) as well as a down-regulation of IKβ. These findings suggest that the secretome of hAMSCs promotes both inflammation and apoptosis in Panc1 pancreatic cancer cells simultaneously.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2025.102411

Authors: Aynaz Khalafi, Fatemeh Safari

Institution: University of Guilan

Language: English

Topic: Mesenchymal stem cell research

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,700