Doxorubicin induces bone loss and modifies multiple cell populations in vivo – Implications for modelling of bone metastasis
Year: 2025
Type: article
Abstract: µCT, a panel of bone marrow cell populations were quantified by flow cytometry and osteoblast/osteoclast numbers were assessed using bone histomorphometry. DOX caused trabecular bone loss, with immunocompetent BALB/c mice being more sensitive to DOX than the immunocompromised BALB/c nude counterparts. The 6 mg/kg dose of DOX altered the ratio of bone marrow immune and haematopoietic cell populations in both groups, increasing the numbers of hematopoietic cells and progenitors, decreasing B cells and increasing the number of neutrophils. Bone marrow macrophage and monocyte numbers were increased following DOX treatment in BALB/c nude mice only. Our data demonstrate that DOX impacts a number of cell types in the bone microenvironment, highlighting the importance of considering treatment-induced bone effects when using DOX in models of bone metastasis.
Source: Journal of bone oncology
Authors: Veli Kaan Aydin, Lubaid Saleh, Penelope Dawn Ottewell, Ingunn Holen
Institutions: Pamukkale University, University of Sheffield
Language: English
Topic: Bone health and treatments
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,990
Funder: University of Sheffield
OpenAlex ID: W7115010034