Solving unique analytical challenges in mRNA-LNP bioprocessing with precise measurement methods
Year: 2025
Type: article
Abstract: UV-Vis spectroscopy is widely used for measuring sample concentrations in gene therapy development, although it poses challenges such as dilution errors and lengthy sample preparation. Variable pathlength technology (VPT), also known as slope spectroscopy, is a UV-Vis-based technique that can be utilized to overcome these hurdles by measuring absorbance changes over variable pathlengths, eliminating dilution and baseline correction requirements. In this article, three case studies are explored to confirm the specificity, accuracy, reproducibility, and linearity of SoloVPE® for encapsulated mRNA analysis.
Source: Nucleic Acid Insights
Author: Nigel Herbert
Institution: Repligen (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7115184330
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