Work

Optimized Method for High-Quality Isolation of Single-Nuclei From Mosquito Fat Body for RNA Sequencing

Year: 2025

Type: article

Abstract: . • We validated this procedure in sugar-fed and blood-fed samples, yielding high-quality single-nucleus RNA sequencing data with high gene counts and low mitochondrial RNA content. • Robust quality metrics allow effective filtering of ambient RNA, enhancing transcriptomic accuracy across different physiological states.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5564

Authors: Stephanie de Carvalho, Colton McNinch, Carolina Barillas-Mury

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: National Institutes of Health

Award: Mosquito Immune Responses and Malaria Transmission