Work

Loss of the distal-proximal GLP-1/Notch activation gradient in the aging C. elegans germline

Year: 2025

Type: preprint

Abstract: In C. elegans hermaphrodites , the distal tip cells (DTCs) capping the distal gonad arms provide the niche signal, a Notch ligand, to maintain germline stem cell pools. Using fixed germlines, it was recently shown that the transcription of a Notch target gene decreased relatively early-on during adulthood. Here, we used the genetically encoded Notch Sensor Able to detect Lateral Signaling Activity (SALSA) to examine the pattern of GLP-1/Notch activity across the aging distal gonad in vivo . Interestingly, we find that the robust and progressively decreasing distal-proximal Notch activation gradient that is observed in young adults gets entirely lost during aging.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/2025.10.22.683991

Authors: Rustelle Janse van Vuuren, Pier-Olivier Martel, Patrick Narbonne

Institution: Université du Québec à Trois-Rivières

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, Canadian Institutes of Health Research

Awards: Dissecting the homeostatic mechanisms that regulate adult stem cell proliferation, Caenorhabditis Genetics Center

OpenAlex ID: W7115698587

Cites: 22

Cited by: 1

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