Microsatellite markers as a useful tool for species identification and assessment of genetic diversity of the Tilia species in the Czech Republic
Year: 2025
Type: article
Abstract: Diagnostic morphological traits distinguishing Tilia cordata and T. platyphyllos are not always apparent. Precise species identification iscrucial for conservation management and for establishing genetic resources offorest reproductive material. Inthis study, weemployed fifteen microsatellite (SSR) markers toidentify diagnostic loci for reliable species discrimination between T. cordata and T. platyphyllos species and toevaluate the genetic diversity. Atotal of250 trees were sampled from eight natural autochthonous populations ofT. cordata and T. platyphyllos inthe Czech Republic, and additional ten individuals ofT. tomentosa were included for comparison. Markers Tc8 and Tc918 were identified asdiagnostic, exhibiting species-specific alleles for T. cordata and T. platyphyllos. Contrary tothe previously published results, weobtained PCR amplicons atlocus Tc918 with anallele size of226 bp inT. cordata. Ananalysis ofthe fifteen SSR loci revealed ahigh level ofgenetic variability inboth the T. cordata and T. platyphyllos populations. Noclonally identical genotypes were detected across the eight study sites. The genetic differentiation (Fst) was higher between T. platyphyllos and T. tomentosa (0.316) than between T. platyphyllos and T. cordata (0.205). Bayesian clustering further revealed distinct genetic structures for T. cordata, T. platyphyllos, and T. tomentosa.
Authors: Pavlína Máchová, Helena Cvrčková, Olga Trčková, Kateřina Vítová, Johana Pechačová, Václav Buriánek, Petr Maděra, Kateřina Houšková, Tomáš Vyhnánek, Pavel Hanáček
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $609
Funder: Ministerstvo Zemědělství
Award: G1761186770
OpenAlex ID: W7115887386