Work

Design, validation, and application of a 1K liquid chip for genome-wide association analysis in Alpine Merino sheep

Year: 2025

Type: article

Abstract: Because wool is an important animal fiber and mutton is rich in nutrients, fine wool sheep are one of the most economically important livestock. China has been selecting and breeding high-quality fine wool sheep breeds since the 1950s and is the largest processing and consuming country for fine wool sheep. In this study, blood samples were collected from Alpine Merino sheep (AMS), Chinese Merino sheep (CMS), Aohan fine wool sheep (AHS), and Qinghai fine wool sheep (QHS). Genomic DNA was extracted and subjected to genome resequencing. GenoBait technology was used for probe design and site optimization, followed by synthesis of a low-density liquid-phase chip. Finally, 409 AMS were randomly selected to verify the chip. The results showed that a total of 1,012 single-nucleotide polymorphism sites (SNPs) were screened and retained for the synthesis of a low-density liquid chip for genome-wide selection and population genetics structure analysis. This chip can provide a useful tool for genome analysis of fine wool sheep and lay a solid foundation for subsequent breeding work.

Source: Frontiers in Veterinary Science

DOI: 10.3389/fvets.2025.1690580

Authors: Tingting Guo, Chao Yuan, Jianbin Liu, Bohui Yang

Institutions: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs

Language: English

Topic: Genetic and phenotypic traits in livestock

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,801