Streptomyces shinuiensis sp. nov., a Salternamides-Producing Bacterium Isolated from Saltern Sediment
Year: 2025
Type: article
Abstract: Materials and Methods Isolation and StrainsStrain HK10 T was originally isolated from saltern sediment on Shinui Island, Republic of Korea (34°36'48.0"N,126°04'32.5"E)using actinomycete isolation agar [8].Subsequent studies further demonstrated that this Strain HK10 T , previously isolated from saltern sediment and identified as a salternamides-producing bacterium, was taxonomically characterized in the present study.The strain is a Gram-stain-positive, aerobic, and filamentous actinobacterium.Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HK10 T belongs to the genus Streptomyces and is most closely related to Streptomyces radiopugnans R97 T (99.4% sequence similarity), followed by other Streptomyces species with sequence similarities of ≤98.9%.The draft genome sequence of strain HK10 T was 6.69 Mbp and a DNA G+C content of 72.7%.Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain HK10 T and type strains of the genus Streptomyces were ≤92.7% and ≤53.0%, respectively, which are below the thresholds for species delineation, indicating that strain HK10 T represents a novel species.The major cellular fatty acids were iso-C 16:0 , anteiso-C 15:0 , anteiso-C 17:0 , and iso-C 14:0 .The predominant menaquinone was menaquinone-9 (MK-9).The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol dimannoside.Whole-cell hydrolysates of strain HK10 T contained L,L-diaminopimelic acid and glycine.Based on phylogenetic, genomic, chemotaxonomic, and physiological evidence, strain HK10 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces shinuiensis sp.nov. is proposed.The type of strain is HK10 T (= NBRC 114904 T = KACC 22137 T ).
Institutions: Inha University, Seoul National University
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W7116742739