Work

High level of KMT5C is associated to better prognosis and its knockdown increases proliferation in head and neck cancer

Year: 2025

Type: article

Abstract: Aim The Histone methyl transferase KMT5C/SUV4-20H2 has been described as a promising marker in several types of cancer. We investigated the impact of KMT5C knockdown on cell proliferation capacity as well as its association with clinic pathological features in HNSC patients.Methods The relative level of KMT5C was measured by quantitative real-time PCR in HNSC lineages with high and low levels of SET/I2PP2A protein, and the cell proliferation capacity was evaluated after the knockdown of KMT5C. Furthermore, statistical tests were used to verify the association of KMT5C levels and pathological features in HNSC patients.Results The knockdown of KMT5C decreased the levels of H4K20me2 and miR-137 while upregulating SET/I2PP2A, KI67, p-Rb, and PCNA proteins. The cell proliferation capacity of the HNSC lineage was also increased after the knockdown of KMT5C. Furthermore, the higher KMT5C level is associated with better survival, while a lower KMT5C level is associated with perineural invasion in HNSC patients.Conclusion KMT5C levels regulate targets involved in cell proliferation and represent a potential biomarker for predicting survival and perineural invasion in HNSC.

Source: Epigenomics

DOI: 10.1080/17501911.2025.2603884

Authors: Lucas Oliveira Sousa, Gabriel da Silva, Thaís Moré Milan, Emerson de Souza Santos, Andréia Machado Leopoldino

Institution: Universidade de São Paulo

Language: English

Topic: Head and Neck Cancer Studies

Subfield: Otorhinolaryngology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Centros de Pesquisa, Inovação e Difusão, Fundação Amazônia Paraense de Amparo à Pesquisa