Year: 2025
Type: article
Abstract: OBJECTIVE: Osteoporotic fractures (OPF) and delayed fracture healing (DFH) pose major health challenges for the elderly. The lncRNA SSTR5-AS1 shows abnormal expression in people with low BMD. Understanding how SSTR5-AS1 regulates fracture healing could help address DFH. METHODS: This study detected the expression levels of SSTR5-AS1, miR-193b-3p, osteogenic markers (RUNX2, ALP, BSP, OCN, OPN) mRNA, and apoptosis-related genes (Bcl-2, Bax, Bad) in osteoblasts and serum samples from all participants using RT-qPCR. Logistic regression was used to identify the independent factors influencing fracture healing. Cell proliferation and apoptosis were assessed using CCK-8 assays and flow cytometry, respectively. The binding relationship between SSTR5-AS1 and miR-193b-3p was validated through dual-luciferase reporter assays. RESULTS: SSTR5-AS1 is significantly downregulated in patients with OPF and DFH and negatively regulates the expression level of miR-193b-3p. SSTR5-AS1 is a protective factor for fracture healing. Elevating SSTR5-AS1 levels significantly reduces miR-193b-3p expression, thereby decreasing osteoblast apoptosis, increasing Bcl-2 expression, and decreasing Bax and Bad levels. Furthermore, enhancing SSTR5-AS1 promotes osteoblast proliferation, upregulates RUNX2, ALP, BSP, OCN, and OPN expression, and facilitates osteoblast differentiation. CONCLUSION: SSTR5-AS1 demonstrates significant diagnostic value for OPF and DFH, making it a promising biomarker for these conditions. When overexpressed, SSTR5-AS1 reduces miR-193b-3p levels, promoting osteoblast proliferation and differentiation. This may accelerate fracture healing.
Authors: Zhuan Zuo, Shiwen Li, Bo Gao, Chenchen Zhao, Yongsheng Ma
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,090
OpenAlex ID: W7117140417