Year: 2025
Type: article
Abstract: Background The literature to oxidative stress as a key contributor to neurodegeneration and neuroinflammation in Alzheimer's disease (AD). Consequently, antioxidant therapies can have a lasting impact for improving the quality of life of AD patients. By strengthening the antioxidant defenses, these therapies may prevent or minimize respiratory and cognitive symptoms characteristic of the disease. Thus, we hypothesize that molecular hydrogen is a potential candidate for the prevention and treatment of AD since it is a gas with antioxidant, anti‐apoptotic and anti‐inflammatory effects. Thus, the present study evaluated whether H 2 treatment prevents and/or minimizes the molecular, cognitive and respiratory alterations caused by AD in experimental model for AD. Method We employed a sporadic AD model induced in mice ( n = 17_ by intracerebroventricular streptozotocin (STZ) injections at a dose of 3 mg/kg. The animals were subsequently exposed to 2% hydrogen (H 2 ) for 5 ( n = 5) and 7 days ( n = 7). Learning and memory were evaluated by Barnes Maze. Ventilation and metabolism were measured via plethysmography under normocapnia and hypercapnia (7% CO 2 ). In addition, we evaluated oxidative stress markers, including superoxide dismutase (SOD) and lipid peroxidation (LPO) levels. Result Microinjection of STZ at a dose of 3 mg/kg increased ventilation in normocapnia, but not in hypercapnia. We did not observe changes in O 2 consumption (VO 2 ) and respiratory coefficient (V E /VO 2 ) of STZ and vehicle animals. Likewise, we did no observe changes in body temperature (Tb) in normocapnia and hypercapnia between the groups, as well as no changes in the dosage of SOD and LPO. Our cognition results showed that 7 days exposure to H 2 gas improved learning by 62% in time compared to STZ animals in room air. Conclusion We conclude that the dose of 3 mg/kg promotes cognitive and respiratory changes present in AD, thus becoming a dose for a model of sporadic Alzheimer's in the intermediate and late window of the disease. In addition, treatment with H 2 gas is effective in promoting improvements in the cognition of animals, making it a potential therapeutic candidate for AD.
Source: Alzheimer s & Dementia
Institution: Universidade Estadual Paulista (Unesp)
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,200
OpenAlex ID: W7117158371