Recent trend on sarcoma stem cell reprogramming towards study of disease progression: A systematic review
Year: 2025
Type: review
Study design: Systematic Review
Abstract: Sarcoma refers to a broad type of cancer characterised by the abnormal growth of cells in connective tissues, bone, muscle, and cartilage.Although relatively rare, the survival rate of sarcoma patients is low primarily due to delayed diagnosis or metastasis at first presentation.Highly metastatic sarcoma has been suggested to be attributable to the presence of cancer stem cells (CSCs).Although a minor cell population, the stemness property renders CSCs stem cell-like characteristics.Nevertheless, CSCs presented a promising target towards advances in sarcoma therapy, particularly through stem cell reprogramming.Thus, this systematic review aims to gather existing studies on the methods of stem cell reprogramming that best produce sarcoma cancer stem cells (CSCs), which are crucial for understanding disease progression.An extensive literature search was conducted across four databases: PubMed, Wiley Online Library, Scopus, and ScienceDirect.The data obtained were synthesized and reported according to the following variables: types of cancer cells used for cancer stem cell generation, vector used for delivery of pluripotent genes, and CSCs maintenance medium.This systematic review demonstrated that cell dedifferentiation was independent of the cell sources.Furthermore, the addition of growth factors such as bFGF, EGF, or FGF significantly enhanced the formation of CSCs' spheroids.Most of the studies included in the review utilized a non-viral vector for the delivery of pluripotent gene markers into the cells.the normal cells [7] .Nevertheless, the role of CSCs in promoting malignancies presented a new approach of targeting stem cell differentiation pathways towards malignancy reversal, with manipulation of specific proteins involved in the reprogramming modulation [8] .Despite limitations on the availability of stem cells, molecular advances have enabled the induction of pluripotency in human somatic cells through the expression of specific pluripotent transcription factors [9] .A study by Yu et al. [10] reported a successful dedifferentiation of human adult fibroblast cells into pluripotent stem cells using Thomason's cluster of transcription factors combined with the transcription factors [10] .The following studies have demonstrated numerous reprogramming methods utilising different aspects of cell reprogramming, such as transcription factor cocktails, gene delivery, and growth factors used for inducing cancer stem cells, as well as media composition for maintaining CSC growth.Nevertheless, the various reprogramming methods presented inconsistent results attributable to different levels of method reproducibility.
Institutions: Universiti Teknologi MARA System, Universiti Teknologi MARA
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $380
OpenAlex ID: W7117248606
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