Investigating the effect of mesenchymal stem cells on the proliferation phase of duodenal wound healing
Year: 2025
Type: article
Abstract: Background: Duodenal perforation represents a critical gastrointestinal surgical emergency, often associated with high rates of morbidity and mortality.Mesenchymal stem cells (MSCs) have the potential to enhance wound healing by releasing various growth factors and antiinflammatory cytokines.This study aims to evaluate and analyze the impact of MSCs on the healing process of duodenal perforation wounds.Method: MSCs were extracted from the umbilical cords of rats and injected into the site of duodenal perforation at two different doses: 1.5 x 10 6 for Treatment Group 1 and 3 x 10 6 for Treatment Group 2. The control group consisted of rats with duodenal perforation that received local injections on normal saline.The levels of Transforming Growth Factor β (TGF-β) and Alpha Smooth Muscle Actin (α-SMA) were assessed via Western Blot analysis, while collagen and fibroblast presence were evaluated through histopathological examination.These examinations were conducted on days 3 and 7 post-treatment.Statistical analysis was performed using SPSS 25.0, with significance defined at p<0.05.Results: Significant increases in the expression of TGF-β, fibroblasts, collagen, and α-SMA were observed in the treatment groups compared to the control group on both day 3 and day 7. Conclusion: The administration of MSCs significantly enhances the proliferation phase of duodenal wound healing through the increased expression of fibroblasts, collagen, TGF-β, and α-SMA cell proliferation and angiogenesis, producing extracellular matrix components, and suppressing inflammation.Key angiogenic factors influenced by MSCs include fibroblasts, collagen, Transforming Growth Factor β (TGF-β), and Alpha Smooth Muscle Actin (ɑ-SMA) [2] .Additionally, MSCs can strengthen the anastomotic line in gastrointestinal surgeries, enhancing tissue integrity and reducing the risk of post-operative leaks.By promoting collagen deposition and decreasing localized inflammation, MSCs help reinforce sutured areas and thus lower the likelihood of leakage [3] .In emergency situations where timely surgical interventions are critical, MSCs offer a valuable strategy for improving surgical outcomes.Their characteristics make them not only crucial for wound healing but also for preventing lifethreatening complications such as anastomotic leaks [4] .This study aims to investigate the role of MSCs in the proliferative phase of duodenal perforation wound healing by assessing the number of fibroblasts, collagen density, the expression of TGF-β and ɑ-SMA.
Authors: Eko Setiawan, Dimas Irfan Nabih
Institution: Sultan Agung Islamic University
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $380
OpenAlex ID: W7117259678
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