Figure 4 from Periostin Promotes Sarcoma Growth by Promoting Tumor-Associated Macrophage Migration and Differentiation
Year: 2025
Type: other
Abstract: Postn recruits bone marrow monocytes and promotes the accumulation of macrophages. A, Quantification of POSTN levels in the serum of sarcoma-bearing mice compared with healthy mice. Each dot represents one mouse. B, Quantification of POSTN levels in the bone marrow of sarcoma-bearing mice compared with healthy mice. Each dot represents one mouse. C, Transwell migration assays to measure the capability of bone marrow–derived monocytes to migrate toward recombinant POSTN protein after 16 hours of exposure or (D) in the presence of POSTN-neutralizing antibodies. E, Transwell migration assays to assess the capability of bone marrow–derived monocytes to migrate toward supernatant from sarcoma cells expressing wild-type (Postn-WT) or enhanced Postn levels (Postn-OE). F and G, Analysis of monocytes (Ly6C+F4/80− cells) and macrophages (Ly6C-F4/80+ cells) at two different time points (days 4 and 6) in culture with recombinant POSTN protein. Unless otherwise indicated, results are presented as mean ± SEM, and P values are derived from a two-tailed unpaired Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). For C–G, each dot represents BMDM derived from a single mouse (n = 4 independent mice). [Created in BioRender. Guarnerio, J. (2025) https://BioRender.com/fo00cpj.]
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7117316990