Figure 3 from Periostin Promotes Sarcoma Growth by Promoting Tumor-Associated Macrophage Migration and Differentiation
Year: 2025
Type: other
Abstract: Postn silencing in tumor cells shapes the sarcoma immune microenvironment. A, Flow cytometry analysis of CD45+ immune cells and different types of immune cells within the CD45+ population in the microenvironment of Postn-WT (shSCR) and Postn-silenced (shPostn) tumors (p53KOVgll3OE). Each dot represents one mouse. B, Subclustering of the mononuclear phagocyte compartment (macrophages, monocytes, and dendritic cells) from scRNA-seq. C, Proportions of the subclusters from B in individual shSCR or shPostn tumors (n = 5 mice in each group). D, Pathways enriched in mononuclear phagocytes from shPostn tumors (top) or from shSCR tumors (bottom). E, Relative expression of IFN-stimulated and proinflammatory genes in CD11b+ myeloid cells FACS-sorted from mouse tumors, generated by either Postn-WT (shSCR) or Postn-silenced (shPostn) sarcoma cells. Each dot represents one mouse. F, Flow cytometry analysis of CD45+ immune cells and different types of immune cells within the CD45+ population in the microenvironment of mice treated with POSTN-blocking antibody (anti-POSTN). Each dot represents one mouse. Unless otherwise indicated, results are presented as mean ± SEM, and P values were derived from a two-tailed unpaired Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Experiments were performed in the p53KOVgll3OE model.
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7117322808