Protocol to study bacterial coaggregation by integrating visual, turbidimetry, microscopy, and thermodynamic analysis
Year: 2025
Type: article
Abstract: Bacterial coaggregation is the specific recognition and adhesion of genetically distinct bacteria, and it is central to multispecies biofilm formation. Here, we present a protocol integrating visual, turbidimetry, epifluorescence microscopy, and thermodynamic analysis to study coaggregation. We describe procedures for performing coaggregation assays and analyzing complementary data from different assays. This integrated workflow provides a robust and multidimensional framework for investigating bacterial coaggregation in different contexts. For complete details on the use and execution of this protocol, please refer to Afonso et al. 1 , 2 • Steps for setting up bacterial suspensions and controls for coaggregation analysis • Procedures for visual and turbidimetric evaluation of coaggregation • Guidance on performing epifluorescence microscopy for aggregate observation • Instructions for measuring contact angles and calculating co-adhesion energies Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Bacterial coaggregation is the specific recognition and adhesion of genetically distinct bacteria, and it is central to multispecies biofilm formation. Here, we present a protocol integrating visual, turbidimetry, epifluorescence microscopy, and thermodynamic analysis to study coaggregation. We describe procedures for performing coaggregation assays and analyzing complementary data from different assays. This integrated workflow provides a robust and multidimensional framework for investigating bacterial coaggregation in different contexts.
Source: STAR Protocols
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W7117330316