Year: 2025
Type: article
Abstract: We studied fine particulate matter (PM) effects on porcine granulosa, a model of endocrine reproductive cells, and on ovarian endothelial cells. PM produced a significant decrease (p < 0.05) in metabolic activity. PM reduced endothelial cell proliferation (p < 0.001). As for redox status, superoxide anion (O2−) production by granulosa was reduced (p < 0.05) by PM, while it was increased in endothelial cells (p < 0.001). PM increased (p < 0.001) nitric oxide (NO) levels in granulosa, while in endothelial cells, it displayed a biphasic effect (p < 0.05) as well as for superoxide dismutase activity (SOD) in granulosa cells (p < 0.001). In endothelial cells, PM increased (p < 0.001) SOD. A reduction in Ferric Reducing Ability of Plasma (FRAP) (p < 0.01) was observed. In granulosa, PM did not induce oxidative damage to DNA, while in endothelial cells, it determined a reduction (p < 0.05). PM significantly inhibited steroidogenesis (p < 0.05). The accumulation of autophagic vacuoles and Vascular Endothelial Growth Factor (VEGF) production was increased (p < 0.05) by PM. The data obtained likely demonstrate that PM induces critical effects on ovarian cells.
Source: Animals
DOI: 10.3390/ani16010081
Institution: University of Parma
Language: English
Subfield: Agronomy and Crop Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,896
Funder: Università degli Studi di Parma
OpenAlex ID: W7117489398