Work

Missing steps of mitochondrial translation initiation identified in plants

Year: 2025

Type: preprint

Abstract: Translation initiation, the first step of the translation cycle, involves initiation factors (mtIF) 2 and 3 in mitochondria. mtIF3 release from the ribosomal small subunit was believed to be a prerequisite for the recruitment of the initiator tRNA. Here we use cryogenic electron microscopy to characterize plant mitochondria pre-initiation complexes and to reveal how plant mtIF3 binds the initiator tRNA and facilitates its accommodation in the decoding center.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2025.12.30.697032

Authors: Vasileios Skaltsogiannis, Heddy Soufari, Philippe Giegé

Institutions: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, Université de Strasbourg, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Paris-Saclay, Synchrotron SOLEIL, Institut de Biologie Intégrative de la Cellule

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Universität Basel, Equipex, Agence Nationale de la Recherche, Université de Strasbourg, Centre National de la Recherche Scientifique, French Infrastructure for Integrated Structural Biology

Awards: G2247417377, G4094285393, Dynamic And regulation of the MItochondrial translational Apparatus in Arabidopsis, G502948375, G513542429, G6694357955, G6852344480, G7237471465, G7240651115, Expression et coordination des génomes dans les fonctionnements et dysfonction-nements mitochondriau, National instrumentation in cryo electron microscopy, Protein-only RNase P as a central regulator of tRNA homeostasis in Arabidopsis nuclei

OpenAlex ID: W7117648492

Cites: 31

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