Synergistic Effect of 5-Fluorouracil and Amphotericin B in Murine Paracoccidioidomycosis: Immune Modulation and Enhanced Fungal Clearance
Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Paracoccidioidomycosis (PCM) is a systemic fungal infection that primarily affects the lungs. Previous studies have shown that 5-fluorouracil (5-FU), a chemotherapeutic agent, reduces pulmonary myeloid-derived suppressor cells (MDSCs), thereby stimulating immune responses in PCM. This study aimed to evaluate the efficacy of the combined 5-FU and AmB therapy in a murine model of PCM. C57BL/6 mice were infected with Paracoccidioides brasiliensis and treated with AmB and/or 5-FU. We found that the 5-FU and AmB combination therapy led to improved disease control, as evidenced by reduced fungal burden, decreased tissue damage, and an increased survival rate. Moreover, the combined treatment was associated with decreased lymphocyte and neutrophil counts, along with an increased number of macrophages in pulmonary tissue, suggesting a controlled infectious process without hyperinflammatory reactions. These findings support the potential of combining 5-FU with conventional antifungal therapy as a promising strategy for enhancing PCM treatment outcomes.
Source: ACS Infectious Diseases
Institutions: Universidade Federal de São Paulo, Virginia Tech
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: G2269311856, G2763057283, G295657101, Association of antifungals with inhibitory mechanisms of immunosuppression in Paracoccidioidomycosis: depletion of Myeloid-derived Suppressor Cells (MDSCs) and inhibition of PD-1 and CTLA-4 checkpoint receptors., G3690646728, G5741552514, Multi-User Equipment approved in grant 2018/14762-3: flow cytometer FACS lyric
OpenAlex ID: W7117679153