Solid phase extraction using a semi-covalent imprinted poly(3-isopropenyl-α,α-dimethylbenzylamine) coupled to batch injection analysis with amperometric detection for tetracyclines determination in milk samples at ultratrace levels
Year: 2025
Type: article
Abstract: ABSTRACT A novel electroanalytical method for tetracyclines (TC) determination in milk samples at ultratrace levels by integrating molecularly imprinted solid-phase extraction (MISPE) with a batch injection analysis (BIA) system using an AuNPs-modified screen-printed carbon electrode is described. In the MISPE procedure, 50.0 mL of sample was preconcentrated using 100.0 mg of the imprinted polymer at pH 5.0, and the analytes were subsequently eluted with 3.0 mL of MeOH containing 0.5% HAc. Preconcentrated TC was injected into the BIA system, and amperometric measurements at AuNPs-modified screen-printed carbon electrode were recorded at 1.2 V vs Ag/AgCl. Linear response in the concentration range of 11 to 300 nmol L⁻¹ (R² = 0.993) was obtained, with limits of detection and quantification of 3 and 11 nmol L⁻¹, respectively. For concentrations of 30 and 100 nmol L⁻¹, intra-day (n = 6) and inter-day (n = 2) precision showed RSD (relative standard deviation) values of 1.71–3.78% and 2.81–4.38%, respectively. Tetracycline and structurally related compounds (oxytetracycline and chlorotetracycline) were determined in milk samples, and the accuracy of the method was confirmed by satisfactory recovery values (93–102%) when compared with High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) results.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,450
OpenAlex ID: W7117752203