Work

Viral transduction for T cell engineering in immunotherapy

Year: 2026

Type: article

Abstract: Viral transduction of primary T cells enables stable genetic engineering for research and immunotherapy, supporting both transgene overexpression and gene deletion. Although the overall workflow can be similar to transduction in other mammalian cell lines, primary T cell culture imposes distinct requirements such as cell-state-dependent nuances shaped by T cell activation and proliferation, which can make it challenging to obtain a sufficient number of genetically engineered T cells. This article provides practical guidance for researchers new to T cells but familiar with basic mammalian cell culture.

Source: Molecules and Cells

DOI: 10.1016/j.mocell.2026.100311

Authors: Yikhyeon Seo, Jimin Pak, Jiyun Han, Joonbeom Bae, Soo Seok Hwang

Institutions: Seoul National University, Korea University

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,500